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KMID : 0545120100200030525
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 3 p.525 ~ p.531
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Production of β-Carotene-Enriched Rice Bran Using Solid-State Fermentation of Rhodotorula glutinis
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Roadjanakamolson M.
Suntornsuk Worapot
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Abstract
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This work was aimed at utilizing rice bran as a substrate for β-carotene production by Rhodotorula glutinis DM 28 under optimized conditions of solid-state fermentation. The biomass and β-carotene content of Rhodotorula glutinis DM 28 grown on rice bran as a sole substrate under solidstate fermentation were 54 g/kg rice bran and 1.65 mg/kg rice bran, respectively. Its biomass and β-carotene content, however, could be improved by 60% and 30%, respectively, using the Central Composite Design for the optimization of its cultivation conditions. The optimized conditions obtained were a pH of 5, a moisture content of 70% (w/w), and a carbon-to-nitrogen ratio of 4. Under these conditions, rice bran containing R. glutinis DM 28 had nutritional values of β-carotene, protein, and fat higher than those of rice bran alone. Yeast-grown rice bran could be suitable, therefore, to use as a β-carotene-enriched supplement in animal feeds.
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KEYWORD
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β-Carotene, Rhodotorula glutinis, rice bran, animal feed, optimization
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